Advanced Applications of ELISA
Expert-defined terms from the Masterclass Certificate in ELISA Assays course at London School of Business and Administration. Free to read, free to share, paired with a professional course.
Absorbance refers to the amount of light absorbed by a substance, in the context… #
Related terms include optical density, which is a measure of the amount of light absorbed by a substance. The concept of absorbance is crucial in ELISA assays as it allows researchers to quantify the amount of analyte present in a sample. For example, in a sandwich ELISA, the amount of enzyme-substrate reaction product is directly proportional to the amount of analyte present in the sample.
Additives are substances added to ELISA assays to enhance the sensitivity… #
Related terms include blocking agents, which are used to prevent non-specific binding of antibodies to the plate. The concept of additives is important in ELISA assays as they can improve the performance of the assay. For example, the use of Tween 20 as a detergent can help to reduce non-specific binding and improve the sensitivity of the assay.
Analyte is the substance being measured in an ELISA assay #
Related terms include antigen, which is the substance that the antibody binds to. The concept of analyte is central to ELISA assays as it is the substance that the assay is designed to detect. For example, in a sandwich ELISA, the analyte is the substance that is bound by the capture antibody and detected by the detection antibody.
Antibody is a protein produced by the immune system in response to the presence… #
Related terms include immunoglobulin, which is another term for antibody. The concept of antibody is crucial in ELISA assays as they are used to detect and quantify the analyte. For example, in a sandwich ELISA, the capture antibody is used to bind the analyte, while the detection antibody is used to detect the bound analyte.
Antigen is a substance that the immune system recognizes as foreign and mounts a… #
Related terms include immunogen, which is a substance that induces an immune response. The concept of antigen is important in ELISA assays as it is the substance that the antibody binds to. For example, in a sandwich ELISA, the antigen is the substance that is bound by the capture antibody and detected by the detection antibody.
Assay is a laboratory test used to detect and quantify a specific substance #
Related terms include test, which is another term for assay. The concept of assay is central to ELISA assays as it is the laboratory test used to detect and quantify the analyte. For example, in a sandwich ELISA, the assay is used to detect and quantify the amount of analyte present in a sample.
Blocking is a step in the ELISA assay protocol where the plate is coated… #
Related terms include blocking agent, which is the substance used to block non-specific binding. The concept of blocking is important in ELISA assays as it helps to reduce non-specific binding and improve the specificity of the assay. For example, the use of bovine serum albumin as a blocking agent can help to reduce non-specific binding and improve the sensitivity of the assay.
Capture antibody is the antibody used to bind the analyte in a sandwich ELISA… #
Related terms include coating antibody, which is another term for capture antibody. The concept of capture antibody is crucial in sandwich ELISA assays as it is used to bind the analyte and allow it to be detected by the detection antibody. For example, in a sandwich ELISA, the capture antibody is used to bind the analyte, while the detection antibody is used to detect the bound analyte.
Conjugate is a complex of an enzyme and an antibody, used to detect the analyte… #
Related terms include enzyme-linked antibody, which is another term for conjugate. The concept of conjugate is important in ELISA assays as it allows the detection of the analyte through the enzyme-substrate reaction. For example, in a sandwich ELISA, the conjugate is used to detect the bound analyte through the enzyme-substrate reaction.
Detection antibody is the antibody used to detect the analyte in a sandwich E… #
Related terms include secondary antibody, which is another term for detection antibody. The concept of detection antibody is crucial in sandwich ELISA assays as it is used to detect the bound analyte and allow it to be quantified. For example, in a sandwich ELISA, the detection antibody is used to detect the bound analyte, while the capture antibody is used to bind the analyte.
ELISA is a laboratory test used to detect and quantify a specific substance, typ… #
Related terms include enzyme-linked immunosorbent assay, which is another term for ELISA. The concept of ELISA is central to the field of immunology as it provides a sensitive and specific method for detecting and quantifying proteins. For example, in a sandwich ELISA, the ELISA is used to detect and quantify the amount of analyte present in a sample.
Enzyme is a protein that catalyzes a specific chemical reaction, used in ELIS… #
Related terms include biocatalyst, which is another term for enzyme. The concept of enzyme is important in ELISA assays as it allows the detection of the analyte through the enzyme-substrate reaction. For example, in a sandwich ELISA, the enzyme is used to catalyze the conversion of a substrate into a product, which is then detected by the assay.
Enzyme #
substrate reaction is a chemical reaction catalyzed by an enzyme, used in ELISA assays to detect the analyte. Related terms include enzyme-catalyzed reaction, which is another term for enzyme-substrate reaction. The concept of enzyme-substrate reaction is crucial in ELISA assays as it allows the detection of the analyte through the conversion of a substrate into a product. For example, in a sandwich ELISA, the enzyme-substrate reaction is used to detect the bound analyte through the conversion of a substrate into a product.
Horseradish peroxidase is an enzyme commonly used in ELISA assays to dete… #
Related terms include HRP, which is an abbreviation for horseradish peroxidase. The concept of horseradish peroxidase is important in ELISA assays as it provides a sensitive and specific method for detecting the analyte. For example, in a sandwich ELISA, horseradish peroxidase is used to catalyze the conversion of a substrate into a product, which is then detected by the assay.
Immune response is the response of the immune system to the presence of a foreig… #
Related terms include immunological response, which is another term for immune response. The concept of immune response is central to the field of immunology as it provides a mechanism for the body to defend against infection and disease. For example, in a sandwich ELISA, the immune response is used to detect and quantify the amount of analyte present in a sample.
Immunoglobulin is a protein produced by the immune system in response to the pre… #
Related terms include antibody, which is another term for immunoglobulin. The concept of immunoglobulin is crucial in ELISA assays as it provides a sensitive and specific method for detecting and quantifying proteins. For example, in a sandwich ELISA, immunoglobulin is used to bind the analyte and allow it to be detected by the detection antibody.
Incubation is a step in the ELISA assay protocol where the plate is incub… #
Related terms include reaction time, which is the time allowed for the reaction to occur. The concept of incubation is important in ELISA assays as it allows the reaction to occur and the analyte to be detected. For example, in a sandwich ELISA, the incubation step is used to allow the capture antibody to bind the analyte and the detection antibody to detect the bound analyte.
Inhibition is a step in the ELISA assay protocol where a substance is add… #
Related terms include inhibitor, which is the substance used to inhibit the reaction. The concept of inhibition is important in ELISA assays as it provides a mechanism for controlling the reaction and improving the specificity of the assay. For example, in a sandwich ELISA, inhibition is used to control the reaction and prevent non-specific binding.
Microplate is a plate with multiple wells used in ELISA assays to detect… #
Related terms include microtiter plate, which is another term for microplate. The concept of microplate is central to ELISA assays as it provides a convenient and efficient method for detecting and quantifying the analyte. For example, in a sandwich ELISA, the microplate is used to detect and quantify the amount of analyte present in a sample.
Non #
specific binding is the binding of antibodies to substances other than the analyte. Related terms include background binding, which is another term for non-specific binding. The concept of non-specific binding is important in ELISA assays as it can reduce the specificity and sensitivity of the assay. For example, in a sandwich ELISA, non-specific binding can be reduced by using blocking agents and optimizing the assay conditions.
Optical density is a measure of the amount of light absorbed by a substance #
Related terms include absorbance, which is another term for optical density. The concept of optical density is crucial in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the optical density is used to detect and quantify the amount of analyte present in a sample.
Plate is a flat dish with multiple wells used in ELISA assays to detect a… #
Related terms include microplate, which is another term for plate. The concept of plate is central to ELISA assays as it provides a convenient and efficient method for detecting and quantifying the analyte. For example, in a sandwich ELISA, the plate is used to detect and quantify the amount of analyte present in a sample.
Reagent is a substance used in an ELISA assay to detect and quantify the… #
Related terms include chemical reagent, which is another term for reagent. The concept of reagent is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the reagent is used to detect and quantify the amount of analyte present in a sample.
Sensitivity is the ability of an ELISA assay to detect small amounts of t… #
Related terms include limit of detection, which is the smallest amount of analyte that can be detected by the assay. The concept of sensitivity is crucial in ELISA assays as it provides a mechanism for detecting and quantifying small amounts of the analyte. For example, in a sandwich ELISA, the sensitivity of the assay is important for detecting small amounts of the analyte in a sample.
Specificity is the ability of an ELISA assay to detect only the analyte a… #
Related terms include selectivity, which is another term for specificity. The concept of specificity is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte without interference from other substances. For example, in a sandwich ELISA, the specificity of the assay is important for detecting only the analyte and not other substances in the sample.
Substrate is a substance that is converted into a product by an enzyme #
Related terms include enzyme substrate, which is another term for substrate. The concept of substrate is crucial in ELISA assays as it provides a mechanism for detecting and quantifying the analyte through the enzyme-substrate reaction. For example, in a sandwich ELISA, the substrate is used to detect and quantify the amount of analyte present in a sample.
Tween 20 is a detergent commonly used in ELISA assays to reduce non #
specific binding. Related terms include detergent, which is another term for Tween 20. The concept of Tween 20 is important in ELISA assays as it provides a mechanism for reducing non-specific binding and improving the specificity of the assay. For example, in a sandwich ELISA, Tween 20 is used to reduce non-specific binding and improve the sensitivity of the assay.
Washing is a step in the ELISA assay protocol where the plate is washed t… #
Related terms include wash step, which is another term for washing. The concept of washing is important in ELISA assays as it provides a mechanism for removing excess reagents and preventing non-specific binding. For example, in a sandwich ELISA, the washing step is used to remove excess reagents and prevent non-specific binding.
Well is a small depression in a plate used in ELISA assays to detect and… #
Related terms include microplate well, which is another term for well. The concept of well is central to ELISA assays as it provides a convenient and efficient method for detecting and quantifying the analyte. For example, in a sandwich ELISA, the well is used to detect and quantify the amount of analyte present in a sample.
Western blot is a laboratory test used to detect and quantify a specific protein #
Related terms include immunoblot, which is another term for Western blot. The concept of Western blot is important in the field of immunology as it provides a sensitive and specific method for detecting and quantifying proteins. For example, in a Western blot, the protein is separated by size and detected using an antibody.
Antigen #
antibody reaction is a chemical reaction between an antigen and an antibody. Related terms include immune complex, which is another term for antigen-antibody reaction. The concept of antigen-antibody reaction is crucial in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the antigen-antibody reaction is used to detect and quantify the amount of analyte present in a sample.
Assay development is the process of designing and optimizing an ELISA ass… #
Related terms include assay design, which is another term for assay development. The concept of assay development is important in ELISA assays as it provides a mechanism for designing and optimizing the assay to detect and quantify the analyte. For example, in a sandwich ELISA, the assay development involves designing and optimizing the capture antibody, detection antibody, and conjugate to detect and quantify the analyte.
Automated ELISA is an ELISA assay that uses automated equipment to… #
Related terms include robotic ELISA, which is another term for automated ELISA. The concept of automated ELISA is important in ELISA assays as it provides a mechanism for high-throughput screening and detection of the analyte. For example, in a sandwich ELISA, the automated ELISA is used to detect and quantify the amount of analyte present in a sample.
Biosensor is a device that uses biological molecules to detect and quantify a sp… #
Related terms include biological sensor, which is another term for biosensor. The concept of biosensor is important in the field of immunology as it provides a sensitive and specific method for detecting and quantifying proteins. For example, in a biosensor, the biological molecules are used to detect and quantify the amount of analyte present in a sample.
Calibration is the process of standardizing an ELISA assay to ensure accu… #
Related terms include standardization, which is another term for calibration. The concept of calibration is important in ELISA assays as it provides a mechanism for ensuring accurate and reliable results. For example, in a sandwich ELISA, the calibration involves standardizing the assay to ensure accurate and reliable results.
Chemiluminescence is a chemical reaction that produces light, used in ELISA</… #
Related terms include luminescence, which is another term for chemiluminescence. The concept of chemiluminescence is important in ELISA assays as it provides a sensitive and specific method for detecting and quantifying the analyte. For example, in a sandwich ELISA, the chemiluminescence is used to detect and quantify the amount of analyte present in a sample.
Colorimetry is a method used to measure the amount of a substance by measuring t… #
Related terms include photometry, which is another term for colorimetry. The concept of colorimetry is important in ELISA assays as it provides a mechanism for measuring the amount of analyte present in a sample. For example, in a sandwich ELISA, the colorimetry is used to measure the amount of analyte present in a sample.
Competition ELISA is an ELISA assay that uses a competitor to comp… #
Related terms include competitive ELISA, which is another term for competition ELISA. The concept of competition ELISA is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a competition ELISA, the competitor is used to compete with the analyte for binding to the antibody, allowing the amount of analyte to be detected and quantified.
Conjugation is the process of attaching a molecule to an antibody or antigen #
Related terms include labeling, which is another term for conjugation. The concept of conjugation is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the conjugation involves attaching an enzyme to the detection antibody, allowing the amount of analyte to be detected and quantified.
Control is a sample used in an ELISA assay to ensure the accuracy and rel… #
Related terms include reference sample, which is another term for control. The concept of control is important in ELISA assays as it provides a mechanism for ensuring the accuracy and reliability of the results. For example, in a sandwich ELISA, the control is used to ensure the accuracy and reliability of the results by comparing the results to a known sample.
Cross #
reactivity is the ability of an antibody to bind to multiple antigens. Related terms include specificity, which is another term for cross-reactivity. The concept of cross-reactivity is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the cross-reactivity of the antibody is important for detecting and quantifying the analyte.
Data analysis is the process of interpreting and analyzing the results of an … #
Related terms include result interpretation, which is another term for data analysis. The concept of data analysis is important in ELISA assays as it provides a mechanism for interpreting and analyzing the results. For example, in a sandwich ELISA, the data analysis involves interpreting and analyzing the results to determine the amount of analyte present in a sample.
Detection limit is the smallest amount of analyte that can be detected by an … #
Related terms include limit of detection, which is another term for detection limit. The concept of detection limit is important in ELISA assays as it provides a mechanism for detecting and quantifying small amounts of the analyte. For example, in a sandwich ELISA, the detection limit is important for detecting small amounts of the analyte in a sample.
Direct ELISA is an ELISA assay that uses a single antibody to dete… #
Related terms include single-antibody ELISA, which is another term for direct ELISA. The concept of direct ELISA is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a direct ELISA, the single antibody is used to detect and quantify the amount of analyte present in a sample.
Dose #
response curve is a graph that shows the relationship between the amount of analyte and the response of the ELISA assay. Related terms include standard curve, which is another term for dose-response curve. The concept of dose-response curve is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the dose-response curve is used to detect and quantify the amount of analyte present in a sample.
Enzyme #
linked immunosorbent assay is a laboratory test used to detect and quantify a specific substance. Related terms include ELISA, which is another term for enzyme-linked immunosorbent assay. The concept of enzyme-linked immunosorbent assay is central to the field of immunology as it provides a sensitive and specific method for detecting and quantifying proteins. For example, in a sandwich ELISA, the enzyme-linked immunosorbent assay is used to detect and quantify the amount of analyte present in a sample.
Fluorescence is a method used to measure the amount of a substance by measuring… #
Related terms include fluorometry, which is another term for fluorescence. The concept of fluorescence is important in ELISA assays as it provides a mechanism for measuring the amount of analyte present in a sample. For example, in a sandwich ELISA, the fluorescence is used to measure the amount of analyte present in a sample.
Immunoassay is a laboratory test used to detect and quantify a specific substanc… #
Related terms include immunological assay, which is another term for immunoassay. The concept of immunoassay is central to the field of immunology as it provides a sensitive and specific method for detecting and quantifying proteins. For example, in a sandwich ELISA, the immunoassay is used to detect and quantify the amount of analyte present in a sample.
Immunochemistry is the study of the chemical properties of immune systems #
Related terms include immunological chemistry, which is another term for immunochemistry. The concept of immunochemistry is important in the field of immunology as it provides a mechanism for understanding the chemical properties of immune systems. For example, in a sandwich ELISA, the immunochemistry is used to understand the chemical properties of the immune system and detect and quantify the analyte.
Immunodetection is the process of detecting and quantifying a specific substance… #
Related terms include immunological detection, which is another term for immunodetection. The concept of immunodetection is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the immunodetection is used to detect and quantify the amount of analyte present in a sample.
Immunoglobulin G is a type of antibody that is commonly used in ELISA ass… #
Related terms include IgG, which is an abbreviation for immunoglobulin G. The concept of immunoglobulin G is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the immunoglobulin G is used to detect and quantify the amount of analyte present in a sample.
Inhibition ELISA is an ELISA assay that uses an inhibitor to inhib… #
Related terms include inhibitory ELISA, which is another term for inhibition ELISA. The concept of inhibition ELISA is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in an inhibition ELISA, the inhibitor is used to inhibit the reaction between the antibody and the analyte, allowing the amount of analyte to be detected and quantified.
Interference is a substance that can interfere with the reaction between the ant… #
Related terms include inhibitor, which is another term for interference. The concept of interference is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the interference can be reduced by using blocking agents and optimizing the assay conditions.
Labeling is the process of attaching a molecule to an antibody or antigen #
Related terms include conjugation, which is another term for labeling. The concept of labeling is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the labeling involves attaching an enzyme to the detection antibody, allowing the amount of analyte to be detected and quantified.
Limit of detection is the smallest amount of analyte that can be detected by an… #
Related terms include detection limit, which is another term for limit of detection. The concept of limit of detection is important in ELISA assays as it provides a mechanism for detecting and quantifying small amounts of the analyte. For example, in a sandwich ELISA, the limit of detection is important for detecting small amounts of the analyte in a sample.
Microplate reader is a device used to measure the amount of light absorbed or em… #
Related terms include plate reader, which is another term for microplate reader. The concept of microplate reader is important in ELISA assays as it provides a mechanism for measuring the amount of analyte present in a sample. For example, in a sandwich ELISA, the microplate reader is used to measure the amount of light absorbed or emitted by the sample.
Optimization is the process of improving the performance of an ELISA assa… #
Related terms include assay optimization, which is another term for optimization. The concept of optimization is important in ELISA assays as it provides a mechanism for improving the performance of the assay. For example, in a sandwich ELISA, the optimization involves improving the sensitivity and specificity of the assay by optimizing the assay conditions.
Quality control is the process of ensuring the accuracy and reliability of the r… #
Related terms include quality assurance, which is another term for quality control. The concept of quality control is important in ELISA assays as it provides a mechanism for ensuring the accuracy and reliability of the results. For example, in a sandwich ELISA, the quality control involves ensuring the accuracy and reliability of the results by using controls and calibration standards.
Reagent quality is the quality of the reagents used in an ELISA assay #
Related terms include reagent purity, which is another term for reagent quality. The concept of reagent quality is important in ELISA assays as it provides a mechanism for ensuring the accuracy and reliability of the results. For example, in a sandwich ELISA, the reagent quality is important for ensuring the accuracy and reliability of the results by using high-quality reagents.
Sensitivity is the ability of an ELISA assay to detect small amounts of t… #
Related terms include limit of detection, which is another term for sensitivity. The concept of sensitivity is important in ELISA assays as it provides a mechanism for detecting and quantifying small amounts of the analyte. For example, in a sandwich ELISA, the sensitivity of the assay is important for detecting small amounts of the analyte in a sample.
Signal amplification is the process of amplifying the signal produced by an E… #
Related terms include signal enhancement, which is another term for signal amplification. The concept of signal amplification is important in ELISA assays as it provides a mechanism for detecting and quantifying small amounts of the analyte. For example, in a sandwich ELISA, the signal amplification involves amplifying the signal produced by the enzyme-substrate reaction, allowing the amount of analyte to be detected and quantified.
Specificity is the ability of an ELISA assay to detect only the analyte a… #
Related terms include selectivity, which is another term for specificity. The concept of specificity is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte without interference from other substances. For example, in a sandwich ELISA, the specificity of the assay is important for detecting only the analyte and not other substances in the sample.
Standard curve is a graph that shows the relationship between the amount of anal… #
Related terms include dose-response curve, which is another term for standard curve. The concept of standard curve is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the standard curve is used to detect and quantify the amount of analyte present in a sample.
Substrate is a substance that is converted into a product by an enzyme #
Related terms include enzyme substrate, which is another term for substrate. The concept of substrate is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the substrate is used to detect and quantify the amount of analyte present in a sample.
Western blotting is a laboratory test used to detect and quantify a specific pro… #
Related terms include immunoblotting, which is another term for Western blotting. The concept of Western blotting is important in the field of immunology as it provides a sensitive and specific method for detecting and quantifying proteins. For example, in a Western blot, the protein is separated by size and detected using an antibody.
X-ray film is a type of film used to detect and quantify the amount of radiation… #
Related terms include radiographic film, which is another term for X-ray film. The concept of X-ray film is important in ELISA assays as it provides a mechanism for detecting and quantifying the amount of analyte present in a sample. For example, in a sandwich ELISA, the X-ray film is used to detect and quantify the amount of analyte present in a sample.
Yield is the amount of product produced by an enzyme #
substrate reaction. Related terms include reaction yield, which is another term for yield. The concept of yield is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the yield is used to detect and quantify the amount of analyte present in a sample.
Z-value is a statistical measure used to evaluate the performance of an ELISA… #
Related terms include signal-to-noise ratio, which is another term for Z-value. The concept of Z-value is important in ELISA assays as it provides a mechanism for evaluating the performance of the assay. For example, in a sandwich ELISA, the Z-value is used to evaluate the performance of the assay and determine the amount of analyte present in a sample.
Antigen presentation is the process by which an antigen is presented to the immu… #
Related terms include antigen processing, which is another term for antigen presentation. The concept of antigen presentation is important in the field of immunology as it provides a mechanism for the immune system to recognize and respond to foreign substances. For example, in a sandwich ELISA, the antigen presentation is important for detecting and quantifying the amount of analyte present in a sample.
Antigen processing is the process by which an antigen is broken down into smalle… #
Related terms include antigen presentation, which is another term for antigen processing. The concept of antigen processing is important in the field of immunology as it provides a mechanism for the immune system to recognize and respond to foreign substances. For example, in a sandwich ELISA, the antigen processing is important for detecting and quantifying the amount of analyte present in a sample.
Antibody affinity is the strength of the binding between an antibody and an anti… #
Related terms include antibody avidity, which is another term for antibody affinity. The concept of antibody affinity is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the antibody affinity is important for detecting and quantifying the amount of analyte present in a sample.
Antibody avidity is the strength of the binding between an antibody and an antig… #
Related terms include antibody affinity, which is another term for antibody avidity. The concept of antibody avidity is important in ELISA assays as it provides a mechanism for detecting and quantifying the analyte. For example, in a sandwich ELISA, the antibody avidity is important for detecting and quantifying the amount of analyte present in a sample.
Assay validation is the process of evaluating the performance of an ELISA … #
Related terms include assay verification, which is another term for assay validation. The concept of assay validation is important in ELISA assays as it provides a mechanism for evaluating the performance of the assay. For example, in a sandwich ELISA, the assay validation involves evaluating the performance of the assay and determining the amount of analyte present in a sample.
Binding affinity is the strength of the binding between a molecule and a recepto… #
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